Instruction of LeJiao Acid-free Fermentation Complex Enzyme（Special for Molasses）

1.  Product introduction

LeJiao acid-free fermentation complex enzyme is an industrial compound preparation obtained by submerged fermentation, extraction and refining of excellent strains. It is a new product specially designed to improve bacteriostatic effect and utilization rate of raw materials, promote yeast growth, reduce harmful waste water discharge and increase alcohol yield.

In terms of alcohol production, sulfuric acid needs to be added in the traditional production process to adjust the PH value to achieve bacteriostatic effect. However, Le Jiao acid-free fermentation complex enzyme can completely replace the use of sulfuric acid, prevent pipe wall scaling caused by the use of sulfuric acid, affect production, and prolong the service life of production equipment, thus achieving the purpose of acid-free fermentation. Meanwhile, it can also promote yeast growth, making yeast stronger in fermentation ability and higher in alcohol production rate. In the later stage of waste water treatment, acid-free fermentation is adopted so that waste water does not contain sulfate ions, greatly reducing the pressure of waste water treatment.

2.  Applicable raw materials

 Applicable to acid-free fermentation of starch alcohol.

3.  Recommended dosage

Based on the mass of raw materials, add according to 20g-30g/ton of raw materials. The suitable PH value can be 3-7and the suitable temperature can be 10℃-60℃.

4.  Application method

Weight the corresponding accelerant according to the amount of raw materials and then directly add it to a tank of distiller's yeast or a saccharifying pot, add the accelerant through the way of flowing after fully stirring and uniformly mixing in the tank. Also, it can be directly added into the No. 1 fermentation tank, fully stirring with aeration.

5.  Storage and expiration date

Solid Powder Type 1KG/Bag, 1*25KG/Barrel Packaging. Store in cool dry place. The quality-guarantee period will be 6 months under 15-25℃ and 12 months under 5-15℃.

6.  Product Specification

This product implements the relevant provisions of the National Food Safety Standards, Food Additives and Enzyme Preparations for Food Industry. The specific product quality standards are as follows:

7.  Safety and Handling Precautions

Enzyme preparations are proteins, which may induce sensitization and cause allergic type reactions in sensitized individuals. Prolonged contact may cause minor irritation for skin, eyes or nasal mucosa, so any direct contiguity with human body should be avoided. If irritation or allergic response for skin or eyes develops, consult a doctor.

Operation norm: Q/LJSWKJ 2-2017

Product Model: LJSWKJ-TM1

Client: Guangxi LeJiao Biomass Energy Co., Ltd.

Trustee: Jiangsu Boli Biological Products Co., Ltd.

Address: No. 93 Yangzhou Road, Jiangyan Economic Development Zone, Jiangyan District, Taizhou City, Jiangsu Province

Food Production License No. SC20132128400753

Advantages of using acid-free fermentation complex enzymes on molasses alcohol

Acid-free fermentation compound enzyme is a new type of high-efficiency biological compound enzyme preparation developed by Guangxi Le Yeh Biotechnology Co., Ltd. to replace the sulfuric acid used in the alcohol production process and realize acid-free fermentation. This product can kill and inhibit the acetic acid bacteria, lactobacillus, staphylococcus aureus and other harmful bacteria that cause acid production in the alcohol production process, but has no effect on the alcohol yeast. It is safe, efficient and environmentally friendly.

Advantages of Leye's acid-free fermentation compound enzyme compared with sulfuric acid.

1、 Environmental and social benefits

1.1 Equipment benefit

The molasses alcohol wastewater produced by the traditional acid adjustment process is highly acidic. It needs to consume 50-60kg of sulfuric acid to produce one ton of alcohol. Because molasses contains a large amount of calcium ions and magnesium ions, these ions ionize with the added sulfuric acid during the molasses production process. The resulting product sulfate radicals will produce a large amount of precipitation, which will adhere to the surface of the equipment and form fouling, which not only affects the thermal efficiency, but also increases the cleaning times of the equipment, which affects the production efficiency and is labor intensive.

The use of acid-free fermentation process completely solves the above problems, because no sulfuric acid is added in the production process of molasses alcohol, and there is no sulfate ion, there will be no precipitation and fouling during the production process; the absence of acid also relieves the fermentation mash. The corrosive effect of the equipment, the acid-free fermentation complex enzyme developed by Guangxi Leye Biotechnology Co., Ltd. is made by fermenting and refining natural peptide extracts with dominant bacteria. It is a food additive and can be used with confidence.

1.2 Environmental benefits

On October 1, 2013, Guangxi’s first local environmental standard "Water Pollution Discharge Standard for Sugarcane Sugar Industry" was officially implemented. The local standard is stricter than the national standard. The traditional process of molasses alcohol production wastewater contains a large amount of sulfate ions. Long-term farmland will cause soil compaction. The sugar content of sugarcane planted from sugarcane fields irrigated with waste water is very low. After a long period of investigation, we found that the sugarcane field produced by waste water from molasses to produce alcohol is higher than that of normal sugarcane fields. At least 8% lower, which has a negative impact on the production of sugar factories, and then formed a vicious circle.

Using an acid-free fermentation complex enzyme to replace sulfuric acid for molasses acid-free fermentation can maintain the molasses alcohol wastewater at its natural pH, significantly reduce the difficulty of processing the fermentation waste liquid, and reduce the corrosion of the fermentation waste liquid on the wastewater treatment device. After the conventional microbial treatment, it will be ready. It can be used as fertilizer to irrigate sugarcane fields, saving energy and environmental protection, and promoting cleaner production.

Fermentation of molasses alcohol wastewater to produce biogas is currently one of the methods for comprehensive utilization of resources. Conventional molasses alcohol wastewater contains a large amount of sulfate ions, which inhibits the growth of methane bacteria and makes the volumetric organic load of the anaerobic reactor quite low, and the output of biogas The content of hydrogen sulfide is high, causing serious odor secondary pollution. The use of acid-free fermentation technology can greatly reduce the sulfate ion in the waste liquid, and reduce it to the concentration below the concentration that does not affect the growth of methane bacteria. The use of anaerobic fermentation to treat the waste liquid can achieve good biogas production, per ton of production The waste liquor of alcohol can produce more than 400m3 of biogas through anaerobic fermentation, which can basically meet the energy required for alcohol production, and the biogas waste can be used as a good organic fertilizer for sugarcane planting, forming a "sugar cane-molasses"

-Alcohol-Biogas-Agricultural fertilizer-Sugarcane" recycling mode to realize the recycling development of comprehensive utilization of resources.

1.3 Safety benefit

Posts where sulfuric acid is added have occupational hazards. As we all know, sulfuric acid is a strong acid, which has a strong irritating and corrosive effect on skin, mucous membranes and other tissues. Inhalation or splashing into the skin can cause severe burns. Prolonged contact with eyes may cause blindness, and It causes pulmonary edema, and the droplets of inorganic acid containing sulfuric acid are carcinogenic. Therefore, adding sulfuric acid threatens the health and safety of job workers.

The acid-free fermentation complex enzyme is used to replace sulfuric acid for the acid-free fermentation of molasses, which is added once per shift in the batching tank. Not only is the amount added is small, but it is also convenient to add, and it will not cause health hazards to the workers.

2、Direct economic benefits

The price of acid-free fermentation complex enzyme is 480 yuan per kilogram, and 20g per ton of raw molasses is added. The trial results at Guangxi Pingguo Baichen Biotechnology Co., Ltd. from December 20th to December 30th, 2018 proved that using this product to achieve acid-free fermentation of molasses, the fermentation pH can be maintained at 4.0- which is more suitable for the growth of alcoholic yeast The range of 4.2 promotes yeast to perform better, speeds up fermentation, effectively reduces reducing sugars, and increases mature mash by more than 0.30.

2.1. Benefit analysis (monthly benefit analysis)

The average liquor content of the mature mash of a biotechnology company in Guangxi was 10.3% (V/V). The mature mash after using the enzyme-free fermentation compound enzyme was 10.6% (V/V), which increased by 0.3%. Contains wine. If the daily production volume of alcohol is 180 tons/day, the monthly production of alcohol is 5,400 tons.

Calculated based on the distillation mature mash feed of 83m³/h, the liquor content increased by 0.3%.

Increase the output of alcohol every day: 83*24*0.3%*0.85=5.0796 tons/day

Monthly increase in alcohol production: 5.0796*30=152.388 tons/month

Calculated at 5300 yuan per ton of alcohol, monthly sales income will increase: 152.388*5300=807656.40 yuan.

2.2. Cost of acid-free fermentation complex enzyme：

The consumption is calculated based on 20 g/ton of molasses, and the estimated consumption of molasses per ton of wine is 4.8 tons. The consumption of acid-free fermentation complex enzyme per ton of wine: 20*4.8=0.096kg, the monthly usage of acid-free fermentation complex enzyme is: 0.096*5400=518.40 kg

Monthly cost of acid-free fermentation compound enzyme: 518.4 kg * 480 yuan = 248,832 yuan ≈ 248,800 yuan

Reduce the amount of sulfuric acid added every month: 5400 tons * 50 kg sulfuric acid / ton wine * 0.6 yuan / kg sulfuric acid = 162,000 yuan

Increased profit: 807656.40-248832+162000=720,824.40 yuan ≈ 720,800 yuan.

3、 Indirect economic benefits

The absence of sulfuric acid in the fermentation process reduces the related costs caused by equipment scaling and corrosion, avoids cumbersome procedures such as filing with relevant departments when purchasing sulfuric acid, greatly improves the working environment of post workers, and promotes the health of post workers And safety.

4、Advantages of Le Yeh Biological Products

4.1 Product functions are stronger

Le Yeh's acid-free fermentation complex enzymes were widely promoted in the market in August 2014 to the present. Le Yeh's acid-free fermentation complex enzymes can not only replace sulfuric acid to achieve acid-free fermentation, but also improve the wine content of mature mash. , The application of many alcohol factories in Yunnan, etc. shows that this product can stably increase the mature mash by more than 0.3 on average. There is no rancidity in the application process, and the product performance is stable after a long time of large-scale production inspection.

4.2 Team technical strength, technical support ability

As early as 2008, the technical team of Le Yeh Biotechnology began to conduct research on enzymes for alcohol production, and successively developed a series of products such as corn alcohol enzymes, yeast promoters and acid-free fermentation complex enzymes. With 8 experienced technicians, we can provide free in-plant technical services according to the requirements of each customer's factory.

4.4 Product stability

Le Yeh's acid-free fermentation complex enzyme has been used in many molasses alcohol plants in Guangxi, Guangdong, Yunnan, Myanmar, Thailand and other molasses alcohol plants for nearly six years. It can completely replace the sulfuric acid adjustment process and acid-free fermentation to ensure stable production.

4.5 Product effect commitment

For molasses that is not severely contaminated (total sugar above 46%, acidity below 12, hammer degree below 88%, no obvious infection can be found under microscope), it is guaranteed to achieve stable acid-free fermentation and mature mash Increase by more than 0.2. For molasses raw materials that meet the above conditions, if the buyer's production loss is caused by the supplier's product quality problems, the supplier will compensate the buyer according to the actual loss caused.  

Guangxi Le Yeh Biological Technology Co., Ltd.   

2020-11-28