LJSW-P40

DESCRIPTION

Glucoamylase LJSW-P40 is derived from Aspergillus niger using submerged fermentation, extraction and refining technology. LJSW-P40 has a high conversion rate of starchy substrates into fermentable sugars. It can hydrolyze α-D-1, 4 glucosidic bonds from the non-reducing end of starch one after another. It can also hydrolyze the α-D-1, 6 glucoside branch bonds of starch and cleaves the α-1, 3 bonds to release glucose. LJSW-P40 is suitable for saccharification in alcohol production.

LJSW-P40 has a robust glucoamylase activity. Use of this product provides higher alcohol yields, faster fermentations, and lower residual starch levels.

PRODUCT CHARACTERISTICS

Declared Enzyme: Glucoamylase

Systematic Name: EC3.2.1.3, 1,4-alpha-D-glucan glucohydrolase

Appearance: Light to dark brown liquid

Activity: 400,000 U/ml (minimum)

Product pH: 3.0 to 5.0

Specific Gravity: 1.10 to 1.20 g/ml

EFFECT OF pH

Effective pH range for LJSW-P40 is 3.0 to 5.5. For maximum activity, the optimal pH is from 3.5 to 4.5. See Figure 1.

EFFECT OF TEMPERATURE

Generally, the temperature range for LJSW-P40 is 30 to 65°C. The optimal temperature range is 55 to 62°C. See Figure 2.

         Figure 1: EFFECT OF PH ON ACTIVITY               Figure 2: EFFECT OF TEMP ON ACTIVITY

         Substrate: 4%(w/v) Soluble Starch                       Substrate: 4%(w/v) Soluble Starch

         Temperature: 60℃                                                pH: 4.2

         Time: 60 min                                                        Time: 60 min     

APPLICATIONS AND BENEFITS

LJSW-P40 is used to saccharify liquified mash from various sources including corn, milo, barley, wheat, rice, potatoes and tapioca. The resultant glucose product is fermented by yeast or another appropriate microorganism to yield alcohol.

LJSW-P40 has been designed to ensure the following benefits for alcohol production:

Increased fermentation rate

Increased alcohol yields

Efficient starch/dextrin degradation

Less residual starch in distiller’s grains

Improved performance in pre-saccharification processes

For ground-grain processing plants using simultaneous saccharification fermentation (SSF), LJSW-P40 may be added directly to the fermenter. For ground-grain dry mill plants and for wet mill plants using a separate pre-saccharification step prior to fermentation, LJSW-P40 may be added to the cooling mash or liquefied starch when the temperature reaches 55 to 62°C. Temperatures above 65°C can result in loss of activity.

DOSAGE GUIDELINES

LJSW-P40 is generally added at a level of 0.30 to 0.36kg/TDS. The actual optimal enzyme dosage level is dependent on the raw materials, temperature, reaction time, and pH of the saccharification/fermentation process, and its desired result at individual plant.

PACKAGING

LJSW-P40 is packaged in 25kg, 30kg food-grade plastic drums or 1125kg IBC totes. Customized packaging is available upon request.

STORAGE

This enzyme has been formulated for optimal stability. Typical shelf-life is six months provided it is stored below 25°C in its original packaging, sealed and unopened, protected from the sunlight. Its shelf-life will be eighteen months if it is stored properly at 0∼10°C. Prolonged storage and/or adverse conditions such as higher temperatures may lead to a higher dosage requirement.

SAFETY & ENZYME HANDLING

Inhalation of enzyme dust and mists should be avoided. In case of contact with the skin or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to the Material Safety Data Sheet.